%A Saleh,A. A. %A El-Hefnawy,S. M. %A Kasemy,Z. A. %A Alhagaa,A. A. %A Nooh,M. Z. %A Arafat,E. S. %D 2022 %J British Journal of Biomedical Science %C %F %G English %K PDR,T2DM,DM,REAL TIME PCR,rETINOPATHY,MIRNA Powered by Editorial Manager® and ProduXion Manager® from Aries Systems Corporation DM,Diabetic retinopathy,MicroRNA,Real-time PCR %Q %R 10.3389/bjbs.2021.10192 %W %L %M %P %7 %8 2022-January-21 %9 Original Research %# %! Mi-RNA-93 and Mi-RNA-152 in the %* %< %T Mi-RNA-93 and Mi-RNA-152 in the Diagnosis of Type 2 Diabetes and Diabetic Retinopathy %U https://www.frontierspartnerships.org/articles/10.3389/bjbs.2021.10192 %V 79 %0 JOURNAL ARTICLE %@ 2474-0896 %X Background and Aim: Diabetes mellitus (DM) is a chronic disorder with diabetic retinopathy (DR) as one of its main microvascular outcomes, being a prime cause of vision loss. Dysregulation of microRNAs (miRNAs) has been associated with some diabetic microvascular complications such as diabetic retinopathy. This hypothesised changes in the serum of miR-93 and miR-152 in diabetes and diabetic retinopathy.Methods: The study cohort consisted of 80 healthy volunteers, 80 type 2 diabetic patients, and 80 diabetic retinopathy patients, of whom 40 had proliferative (PDR) and 40 non-proliferative retinopathy (NPDR). Serum fasting and 2-hour postprandial glucose (2hPP), glycated haemoglobin (HbA1c), fasting insulin, and HOMA-IR were evaluated by routine methods, miR-93 and miR-152 expression by quantitative real-time PCR.Results: FBG, 2hPP, fasting insulin, HOMA-IR, and miR-152 showed an increasing trend across groups while miR-93 showed a decreasing trend (all p < 0.001). Binary logistic regression analysis for prediction of DR found that the most significant were miR-152 (OR 1.37, 95% CI: 1.18–1.58, <0.001), BMI (1.13, [1.07–1.31], p = 0.004), duration of disease (1.29 [1.04–1.6] p = 0.018), and miR-152 (0.01, [0.0–0.47] p = 0.019). The most significant predictors of PDR were miR-152 (OR = 1.47, 95% CI: 1.12–1.92, p = 0.005), HOMA-IR (2.66 [1.30–5.45] p = 0.007), and miR-93 (0.25 [0.07–0.86] p = 0.028).Conclusion: MiR-93 and miR-152 can differentiate patients with diabetes and those with DR. Both miRNAs might be potential biomarkers for diabetes and diabetic retinopathy, and specifically for proliferative diabetic retinopathy.