AUTHOR=NasanOchir Munhkjargal , Uchiyama Akihiko , Amalia Syahla Nisaa , Ishikawa Mai , Nasanbat Bolor , Taivanbat Bayarmaa , Kosaka Keiji , Nishio Mayu , Yokoyama Yoko , Ogino Sachiko , Torii Ryoko , Motegi Sei-ichiro 

TITLE=Human urine-derived stem cells: potential therapy for psoriasis-like dermatitis in mice

JOURNAL=Journal of Cutaneous Immunology and Allergy

VOLUME=Volume 7 - 2024

YEAR=2024

URL=https://www.frontierspartnerships.org/journals/journal-of-cutaneous-immunology-and-allergy/articles/10.3389/jcia.2024.13069

DOI=10.3389/jcia.2024.13069

ISSN=2574-4593

ABSTRACT=Over the past decade, significant advancements in stem cell research led by mesenchymal stem cells (MSCs) have facilitated their practical application in clinical settings, including inflammatory skin diseases. Urine-derived stem cells (USCs) are isolated from autologous urine in a noninvasive approach with properties similar to mesenchymal stem cells (MSCs). However, the therapeutic potential of USCs for inflammatory skin diseases has not yet been fully explored. Herein, we report the therapeutic effects of USCs-derived culture supernatants on mice with psoriasis-like dermatitis using our originally established human USCs model. We examined the isolation of USCs from human urine using a simple centrifugation process. The ability of the cells to adhere to the plastic culture bottles was confirmed. MSCs-like cell surface markers, such as CD29, CD44, CD73, and CD90, were positive in about 80-90% of cells. However, hematopoietic stem cell markers, including CD34, CD45, and HLA-DR, were not expressed by FACS analysis. Differentiation assays showed that these cells have the ability to differentiate into adipocytes, osteocytes, and chondrocytes. USCs-conditioned medium (CM) treatment significantly suppressed the severity of dermatitis in imiquimod (IMQ)-treated psoriasis mice model. Histopathological examination revealed that USCs-CM treatment attenuated epidermal thickness and infiltration of inflammatory cells, such as CD3 + T-cells, MPO + neutrophils, and CD68 + macrophages in dermatitis-affected areas in IMQ-treated psoriasis mice. In summary, our findings revealed new potential strategies for utilizing USCs and USCs-CM as therapeutic agents for inflammatory skin diseases, including psoriasis.