<?xml version="1.0" encoding="utf-8"?>
    <rss version="2.0">
      <channel xmlns:content="http://purl.org/rss/1.0/modules/content/">
        <title>Acta Biochimica Polonica | New and Recent Articles</title>
        <link>https://www.frontierspartnerships.org/journals/acta-biochimica-polonica</link>
        <description>RSS Feed for Acta Biochimica Polonica | New and Recent Articles</description>
        <language>en-us</language>
        <generator>Frontiers Feed Generator,version:1</generator>
        <pubDate>2026-08-05T14:09:28.783+00:00</pubDate>
        <ttl>60</ttl>
        <item>
        <guid isPermaLink="true">https://www.frontierspartnerships.org/articles/10.3389/abp.2026.16122</guid>
        <link>https://www.frontierspartnerships.org/articles/10.3389/abp.2026.16122</link>
        <title><![CDATA[The yields of nucleic acids and proteins extracted from various murine tissue types]]></title>
        <pubdate>2026-07-29T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Yifan Chen</author><author>Xixuan Dong</author><author>Lixiang Xue</author><author>Zhongnan Yin</author>
        <description><![CDATA[ObjectiveTo characterize deoxyribonucleic acid (DNA), ribonucleic acid (RNA), and protein yields extracted from tissue of fourteen mouse organ types to establish baseline yield estimates for these biomolecules and provide guidance for tissue sample usage and storage in biomedical studies.MethodsSix wild-type C57BL/6J and six BALB/c nude mice were euthanized and fresh tissues were collected for extracting total DNA, RNA, and protein. The concentration, purity, and quality of nucleic acids were measured with NanoDrop™ One spectrophotometer, gel electrophoresis or capillary electrophoresis. Protein concentration was determined using the bicinchoninic acid (BCA) method.ResultsDNA yields were highest in the colorectum and lowest in bone. RNA yields were highest in the colorectum and spleen of C57BL/6J and BALB/c nude mice, respectively, and lowest in bone. Protein yields were highest in the kidney and liver of C57BL/6J and BALB/c nude mice, respectively, and lowest in bone. The tissue quantities required to obtain 10 μg of DNA, 10 μg of RNA, and 1 mg of protein were calculated and presented along with corresponding tissue sizes.ConclusionDNA, RNA, and protein yields varied significantly across different mouse tissue types. These findings provide a reference for estimating tissue sample sizes needed for downstream assays and optimizing the preservation of mouse tissues.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontierspartnerships.org/articles/10.3389/abp.2026.17388</guid>
        <link>https://www.frontierspartnerships.org/articles/10.3389/abp.2026.17388</link>
        <title><![CDATA[Correction: Value evaluation of serum (sdLDLc*HCYc)/HDLc ratio in the stability of intracranial arterial plaques in patients with acute cerebral infarction]]></title>
        <pubdate>2026-07-29T00:00:00Z</pubdate>
        <category>Correction</category>
        <author>Hongyu Hao</author><author>Xing Xing</author><author>Yajing Li</author><author>Hongshan Chu</author><author>Lei Zhao</author><author>Siqi Cheng</author><author>Yang Liu</author><author>Tiankui Wang</author><author>Nan Meng</author><author>Ruisheng Duan</author>
        <description></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontierspartnerships.org/articles/10.3389/abp.2026.16877</guid>
        <link>https://www.frontierspartnerships.org/articles/10.3389/abp.2026.16877</link>
        <title><![CDATA[Mitochondrial respiration and nucleotide profiling in human left internal mammary artery from CABG: a novel real-time ex vivo approach]]></title>
        <pubdate>2026-07-27T00:00:00Z</pubdate>
        <category>Brief Research Report</category>
        <author>Alicja Braczko</author><author>Andrzej Łoś</author><author>Marta Piotrowska</author><author>Ada Kawecka</author><author>Iga Walczak</author><author>Mikołaj Krysiak</author><author>Marcin Hellmann</author><author>Maciej Brzeziński</author><author>Ryszard T. Smoleński</author><author>Barbara Kutryb-Zając</author>
        <description><![CDATA[Mitochondrial dysfunction plays a critical role in the pathogenesis of cardiovascular and metabolic diseases. However, direct assessment of mitochondrial respiration in human vascular tissue remains technically challenging. In this study, we present an ex vivo approach for real-time analysis of mitochondrial respiration in human left internal mammary artery (LIMA) grafts obtained during coronary artery bypass grafting (CABG). LIMA segment was collected intraoperatively and processed for bioenergetic assessment using the Seahorse XF Flex 3D Analyzer. Mitochondrial respiration was evaluated using the Mito Stress Test, enabling real-time measurement of oxygen consumption rate (OCR). In parallel, tissue nucleotide levels were quantified using high-performance liquid chromatography (HPLC), allowing complementary assessment of cellular energy status and redox balance. We demonstrate the feasibility of measuring mitochondrial respiration in intact human arterial tissue ex vivo. To our knowledge, this is the first application of the Seahorse XF Flex 3D platform for real-time bioenergetic analysis in intact human vascular tissue. Combined analysis of OCR and nucleotide levels enabled integrated assessment of vascular bioenergetic status. The applied protocol enabled reliable assessment of key bioenergetic parameters, including basal and maximal respiration. This study establishes a novel proof-of-concept workflow for ex vivo bioenergetic profiling of human vascular grafts, providing a platform for future investigations of vascular metabolism in cardiovascular and metabolic disorders.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontierspartnerships.org/articles/10.3389/abp.2026.16436</guid>
        <link>https://www.frontierspartnerships.org/articles/10.3389/abp.2026.16436</link>
        <title><![CDATA[The role of gut microbiome disruption in the development of metabolic dysfunction-associated kidney disease]]></title>
        <pubdate>2026-07-24T00:00:00Z</pubdate>
        <category>Review</category>
        <author>Michał Witkowski</author><author>Jarosław Przybyciński</author><author>Bartosz Wojciuk</author><author>Wiktor Czaja</author><author>Natalia Gołembiewska</author><author>Edyta Gołembiewska</author>
        <description><![CDATA[Metabolic dysfunction-associated kidney disease (MDAKD) is increasingly recognised as a major clinical consequence of the global rise in obesity, type 2 diabetes, hypertension, and cardiovascular disease. Accumulating evidence suggests that the gut microbiota may contribute to the development and progression of metabolic and renal disorders through complex mechanisms involving microbial metabolites, immune activation, and disruption of the intestinal barrier. This review provides an overview of current knowledge regarding the role of the gut microbiota and gut-derived metabolites in the pathogenesis of chronic kidney disease (CKD) associated with metabolic disorders. Special attention is given to short-chain fatty acids, bile acids, N-trimethylamine oxide, branched-chain amino acids, indoxyl sulfate, p-cresol sulfate, and lipopolysaccharides. Accumulating experimental and clinical evidence suggests that dysbiosis may contribute to chronic low-grade inflammation, insulin resistance, endothelial dysfunction, lipotoxicity, and profibrotic signaling pathways associated with kidney injury and cardiovascular complications. The review also identifies significant limitations in current microbiome research, such as the predominance of animal studies, methodological challenges in metabolite quantification, and difficulties in establishing causality in humans. Emerging therapeutic strategies targeting the gut microbiota, including dietary interventions, prebiotics, probiotics, sodium-glucose cotransporter 2 inhibitors, glucagon-like peptide-1 receptor agonists, and faecal microbiota transplantation, may offer novel approaches to slowing CKD progression and improving metabolic health. However, further mechanistic and clinical studies are required to determine the efficacy of microbiota-targeted therapies in MDAKD.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontierspartnerships.org/articles/10.3389/abp.2026.16555</guid>
        <link>https://www.frontierspartnerships.org/articles/10.3389/abp.2026.16555</link>
        <title><![CDATA[Screening performance of the MUS-3600 urine analyzer for urinary tract infection: comparison with urine culture]]></title>
        <pubdate>2026-07-16T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Zeynep Mine Yalçınkaya Kara</author><author>Leyla Genç</author><author>Merve Sena Odabaşı</author><author>Elif Aktaş</author>
        <description><![CDATA[BackgroundUrine culture is the gold standard for diagnosing urinary tract infections (UTIs), but most samples yield negative results. A rapid and accurate screening method could accelerate reporting, reduce workload, and lower costs. This study aimed to evaluate the diagnostic performance of the MUS-3600 urine analyzer as a screening tool for UTIs.MethodsUrine samples submitted to the microbiology laboratory were analyzed using the MUS-3600 within 10 min of collection. The analyzer uses flow-type micro-imaging and artificial intelligence–based particle classification. Culture results with ≥104 CFU/mL were defined as positive; lower counts were negative.ResultsFor white blood cells (WBC, >39/μL), sensitivity, specificity, PPV, NPV, and elimination rate were 63.4%, 84.5%, 50.8%, 90.1%, and 67.5%, respectively. Bacilli (>103/μL) showed 62.1%, 88.1%, 56.9%, 90.2%, and 70.4%. Suspected cocci (>859/μL) demonstrated lower accuracy (44.4%, 78.7%, 34.5%, 84.9%, and 62.8%). Combining WBC and bacilli yielded the highest elimination rate (77.2%) and NPV (87.9%). The analyzer performed better in males (NPV 93.9%) than in females (NPV 82.1%).ConclusionThe MUS-3600 provides moderate accuracy for UTI screening and may help reduce unnecessary urine cultures, particularly in male patients. However, its limited sensitivity, especially for cocci, prevents its use as a stand-alone screening tool. Further studies are needed to confirm its role as a complementary method for culture elimination.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontierspartnerships.org/articles/10.3389/abp.2026.16873</guid>
        <link>https://www.frontierspartnerships.org/articles/10.3389/abp.2026.16873</link>
        <title><![CDATA[Beyond biochemistry: multiparametric ultrasound parameters and their molecular correlates in cardio-renal-metabolic syndrome]]></title>
        <pubdate>2026-07-14T00:00:00Z</pubdate>
        <category>Mini Review</category>
        <author>Marcin L. Kutek</author><author>Jacek M. Witkowski</author>
        <description><![CDATA[Cardio-renal-metabolic syndrome (CRMS)—characterized by the pathological interplay of visceral adiposity, insulin resistance, chronic kidney disease, and cardiovascular disease—affects over 90% of US adults across its staging spectrum, yet its multi-organ burden remains difficult to assess non-invasively at the point of care. This narrative mini review examines whether multiparametric ultrasound within a single examination can serve as an integrated imaging biomarker set complementary to established molecular markers of CRMS. A narrative search of PubMed (2015–2026) was conducted using PICO-structured queries. The hepatic controlled attenuation parameter and liver stiffness measurement correlate directly with Homeostatic Model Assessment of Insulin Resistance (HOMA-IR), CRP, and adipokine dysregulation, with pooled CAP cutoffs of 268–313 dB/m and elastography thresholds of 8.2–13.6 kPa for fibrosis staging in MASLD. Epicardial adipose tissue thickness correlates with circulating IL-17A (r = 0.308), hs-CRP (r = 0.666), and HOMA-IR (r = 0.567–0.580), independently predicting left ventricular diastolic dysfunction beyond BMI. Carotid intima-media thickness tracks eGFR decline longitudinally and predicts cardiovascular mortality in CKD populations. Renal resistive index, with a validated threshold of ≥0.70, independently predicts GFR decline and cardiovascular mortality across diabetic and hypertensive populations. Renal shear wave elastography distinguishes fibrotic from non-fibrotic parenchyma, with 93.1% sensitivity against biopsy. Cross-compartmental correlations and composite imaging-biochemistry models consistently outperform single-parameter approaches. Two critical gaps remain: specific ultrasound values have not been associated with AHA/ACC CKM stages, and no outcome study has validated a multiorgan protocol. Multiparametric ultrasound provides a clinically feasible, evidence-based CRMS assessment that remains to be validated in prospective trials.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontierspartnerships.org/articles/10.3389/abp.2026.16376</guid>
        <link>https://www.frontierspartnerships.org/articles/10.3389/abp.2026.16376</link>
        <title><![CDATA[Divergence in surface protein exposure between reference and clinical-derived Candida glabrata (Nakaseomyces glabratus) strains (CBS138 vs. BG2) – a preliminary proteomic perspective]]></title>
        <pubdate>2026-07-08T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Aneta Bednarek</author><author>Olga Barczyk-Woznicka</author><author>Justyna Karkowska-Kuleta</author><author>Elzbieta Pyza</author><author>Maria Rapala-Kozik</author><author>Dorota Satala</author>
        <description><![CDATA[Candida glabrata (currently classified as Nakaseomyces glabratus) is an opportunistic fungal pathogen notable for its intrinsic antifungal tolerance and ability to persist in host environments. Although strain CBS138 has served as the principal model for genetic and functional studies, accumulating evidence indicates substantial intraspecies diversity that may shape virulence, immune interactions and stress adaptation. In particular, the widely used clinical isolate BG2 differs from CBS138 in genome structure, adhesin regulation and macrophage survival, yet the extent to which these differences are reflected at the fungal cell surface remains unknown. Here, we present a comparative characterization of the surface-exposed proteomes (surfaceomes) of CBS138 and BG2 across three biologically relevant growth conditions: YPD-grown yeast-like cells, RPMI-cultured planktonic aggregates and RPMI-formed biofilms. Using trypsin shaving combined with LC–MS/MS, we identified pronounced strain- and condition-dependent differences in surface protein composition, encompassing adhesins, yapsin proteases and selected moonlighting proteins. Whereas CBS138 showed greater representation of adhesion- and interaction-related surface proteins, BG2 preferentially displayed proteins associated with cell-wall architecture and remodelling, consistent with distinct surface-mediated adaptive strategies. Transmission electron microscopy revealed condition-dependent differences in cell-wall thickness in both strains, with BG2 displaying a broader range of values and the highest thickness under biofilm conditions, providing structural context for variation in protease accessibility and surface-protein detectability. Collectively, our findings highlight substantial surfaceome plasticity in C. glabrata and underscore the importance of considering intraspecies diversity when interpreting host–pathogen interactions and fungal virulence pathways.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontierspartnerships.org/articles/10.3389/abp.2026.16345</guid>
        <link>https://www.frontierspartnerships.org/articles/10.3389/abp.2026.16345</link>
        <title><![CDATA[Mitochondrial morphology and mtDNA content in fibroblasts from patients with different types of mucopolysaccharidosis]]></title>
        <pubdate>2026-06-22T00:00:00Z</pubdate>
        <category>Brief Research Report</category>
        <author>Klaudia Walczak</author><author>Lidia Gaffke</author><author>Karolina Pierzynowska</author><author>Natalia Sowa-Rogozińska</author><author>Ewa Piotrowska</author>
        <description><![CDATA[Mucopolysaccharidosis (MPS) is a group of inherited metabolic diseases, characterized by defects in the degradation of glycosaminoglycans and their accumulation in lysosomes. However, various secondary cellular changes also contribute to the pathomechanism of MPS. Previous studies have reached contradictory conclusions about the changes in mitochondria in MPS, from increased numbers of mitochondria to impaired activities of some mitochondrial respiratory chain enzymes to no changes in mitochondrial respiration. In this preliminary, hypothesis-generating study, mitochondrial network morphology and mitochondrial DNA (mtDNA) abundance were investigated in fibroblasts derived from patients suffering from diverse MPS types. Fluorescence microscopy and real-time PCR were used to estimate these parameters, respectively. No significant changes in the mitochondrial network morphology were detected in MPS fibroblasts relative to control cells. Decreased levels of mtDNA relative to nuclear DNA levels were evident in some (I, II, IIIA, IIID, and VI) but not all MPS types compared to control fibroblasts. The results of this study suggest that there are some, although perhaps not dramatic, impairments of mitochondrial functions in some MPS types; however, they do not provide direct evidence of mitochondrial dysfunction. Therefore, these findings should be interpreted as descriptive and exploratory, highlighting the need for further functional and mechanistic studies.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontierspartnerships.org/articles/10.3389/abp.2026.16706</guid>
        <link>https://www.frontierspartnerships.org/articles/10.3389/abp.2026.16706</link>
        <title><![CDATA[Elemental changes in tooth hard tissues during demineralization and fluoride-induced remineralization: an SEM-EDX study]]></title>
        <pubdate>2026-06-09T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Svanishvili Nino</author><author>Mamaladze Marina</author><author>Mauro Labanca</author><author>Vadachkoria Dea</author>
        <description><![CDATA[BackgroundTooth enamel and dentin are the most highly mineralized tissues in the human body, and their structural stability depends on the balanced content of calcium (Ca), phosphorus (P), and fluoride (F). Demineralization leads to the loss of these ions, weakening the tooth structure and increasing susceptibility to caries.ObjectiveThis study aimed to evaluate elemental changes in Ca, P, and F in intact, demineralized, and remineralized teeth across the enamel, dentin–enamel junction and peripulpal dentin.Materials and methods45 extracted human teeth were longitudinally sectioned to yield 90 paired specimens. One half of each tooth served as a baseline (intact) control, while the corresponding half was subjected to acid-induced demineralization. Following demineralization, the experimental halves were randomly assigned to one of three remineralization protocols (n = 15 per group): fluoride varnish, fluoride toothpaste with rinsing, and fluoride toothpaste without rinsing. Elemental composition was analyzed using scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM-EDX). Statistical analysis included paired t-tests for within-tooth comparisons and one-way ANOVA for treatment effects (p < 0.05).ResultsAt baseline, enamel exhibited significantly higher calcium and phosphorus levels compared to dentin regions. Demineralization resulted in a significant reduction in mineral content, particularly in dentin, along with complete loss of detectable fluoride (p < 0.0001). Remineralization increased calcium and fluoride levels across all groups. Fluoride varnish showed the highest fluoride retention trend, particularly in enamel; however, no statistically significant differences were observed among treatment groups for calcium and phosphorus.ConclusionFluoride-based treatments contributed to partial remineralization of tooth hard tissues. Fluoride varnish showed a tendency toward greater fluoride incorporation, particularly in enamel; however, statistically significant superiority over other treatment approaches was not demonstrated. These findings support the role of fluoride in remineralization while highlighting the need for further clinically relevant studies.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontierspartnerships.org/articles/10.3389/abp.2026.16673</guid>
        <link>https://www.frontierspartnerships.org/articles/10.3389/abp.2026.16673</link>
        <title><![CDATA[The promise and peril of mirror bacteria]]></title>
        <pubdate>2026-05-29T00:00:00Z</pubdate>
        <category>Perspective</category>
        <author>Kyna Rigouts</author><author>Gregor Becker</author>
        <description><![CDATA[Mirror bacteria are organisms constructed from mirror-image biomolecules unlike those used by all known life and are moving from theoretical concept to practical feasibility. Their unusual chemistry that makes them resistant to natural degradation and isolated from ecosystems, fuels optimism for applications such as more stable medicines, long-term information storage, and novel platforms for bioengineering. At the same time, these very characteristics raise reservations: they challenge existing definitions of “life,” expose gaps in regulation, and risk undermining public trust if deployed without careful oversight. This perspective assesses not only those risks but also the opportunities of mirror bacteria, moving beyond narrow biosafety debates to explore their broader ethical, regulatory, and cultural implications. We argue that mirror bacteria represent a rare testcase for how science and society navigate the responsibilities of creating fundamentally new forms of life. Their development offers not only technical possibilities but also a chance to build more resilient frameworks for governance and ethics in synthetic biology.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontierspartnerships.org/articles/10.3389/abp.2026.16599</guid>
        <link>https://www.frontierspartnerships.org/articles/10.3389/abp.2026.16599</link>
        <title><![CDATA[Precise genome modification of agrobacterium based on one-step homologous recombination via endogenous transfer DNA]]></title>
        <pubdate>2026-05-25T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Yu Liu</author><author>Geng Sun</author><author>Chunyan Gao</author><author>Rui Gao</author><author>Jian Tang</author><author>Menghu Wang</author><author>Jian Guo</author><author>Rui Guo</author>
        <description><![CDATA[Here, a simple and precise genome modification method for agrobacterium was described and tested in strain GV3101. Taking the induced endogenous Transfer DNA (T-DNA) as the single-strand homologous repair template, this makes it possible to achieve valid and accurate genome modification for agrobacterium even without the strengthening of gene editing tools such as the CRISPR/Cas9 system. In addition, the introduction of tool plasmids into recipient agrobacteria can be achieved by the freeze-thaw method rather than complex triparental mating or expensive electroporation, which makes it more convenient. Here, the modification rate of agrobacterium strain GV3101 at both the upstream and downstream of VirD2 is 4.5% with 100% sequence accuracy. This research provides a valuable potential tool for genetic engineering in agrobacterium, with potential applications in biotechnology and agricultural sciences.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontierspartnerships.org/articles/10.3389/abp.2026.16545</guid>
        <link>https://www.frontierspartnerships.org/articles/10.3389/abp.2026.16545</link>
        <title><![CDATA[Recommendations of the Polish Zebrafish Society on the use of the zebrafish (Danio rerio) model in biomedical research]]></title>
        <pubdate>2026-05-22T00:00:00Z</pubdate>
        <category>Review</category>
        <author>Piotr Podlasz</author><author>Marta Migocka-Patrzałek</author><author>Tomasz K. Prajsnar</author><author>Anna Sarosiak</author><author>Przemko Tylzanowski</author>
        <description><![CDATA[The zebrafish (Danio rerio) has become a widely adopted vertebrate model in biomedical research, offering high translational value while supporting the principles of Replacement, Reduction, and Refinement (3Rs). Here, the Polish Zebrafish Society presents comprehensive, field-specific recommendations for the responsible and effective use of zebrafish across major areas of biomedical research. The document summarizes the scientific rationale and experimental advantages of zebrafish-based approaches in oncology, toxicology, neurology and neuropsychiatry, metabolic diseases, immunology, cardiology, and genetic disease modelling. The model’s key strengths include rapid development, optical transparency, genetic tractability, and strong conservation of molecular and physiological pathways relevant to human disease. These features enable real-time in vivo analysis of pathological processes, high-throughput pharmacological and toxicological screening, and functional validation of disease-associated genes. By integrating ethical considerations with robust experimental evidence, these recommendations aim to promote standardized implementation of zebrafish models in academic research, pharmaceutical development, and regulatory science. Broad adoption of zebrafish-based approaches can accelerate preclinical discovery, enhance translational relevance, and substantially reduce reliance on higher vertebrate models.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontierspartnerships.org/articles/10.3389/abp.2026.16571</guid>
        <link>https://www.frontierspartnerships.org/articles/10.3389/abp.2026.16571</link>
        <title><![CDATA[Pilot analysis of protein profile alterations in plasma and aortic tissue of spontaneously hypertensive rats]]></title>
        <pubdate>2026-05-21T00:00:00Z</pubdate>
        <category>Brief Research Report</category>
        <author>Anastasios Papageorgiou</author><author>Fragkiski-Ioanna Sofiou</author><author>Anastassios Philippou</author><author>Maria Gkrampovari</author><author>Konstantinos Agiannitopoulos</author><author>Lubomir Traikov</author>
        <description><![CDATA[The role of proteins in cellular activity has received close attention. Proteome analysis can detect early enough changes in the cell before the appearance of pathological changes. Due to the increasing number of cardiovascular complications, more specifically hypertension in modern life, this experimental pilot study tries to detect early changes in the protein profile in cases with arterial hypertension. In this pilot study, animal models were used, specifically control rats and spontaneously hypertensive rats (SHRs), which are widely used as translational models relevant to human cardiovascular physiology. Using gel electrophoresis (GE) method, we analysed proteomic signature of blood plasma and aorta tissue samples of the animal models.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontierspartnerships.org/articles/10.3389/abp.2026.15045</guid>
        <link>https://www.frontierspartnerships.org/articles/10.3389/abp.2026.15045</link>
        <title><![CDATA[Skin examination in extreme conditions]]></title>
        <pubdate>2026-05-01T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Joanna Igielska-Kalwat</author>
        <description><![CDATA[Extreme indoor and outdoor conditions in patients’ places of stay (hot or cold air and changes in humidity) lead to weakening of the lipid barrier function and disproportionate epidermal microbiota which results in a lower threshold of physical and chemical tolerance of the skin manifesting as erythema, burning, dryness and deterioration of skin condition. Chronic epidermal irritation caused by the above factors leads to discomfort and prevents the proper functioning of the skin, as it results in thickening of the epidermis, widening of the sebaceous glands and pigmentation disorders. Regenerative mechanisms preventing lipid peroxidation or carbonylation of skin cell proteins, including epidermis, are focused on restoring physiology and not on “fibroblast protection”, which can accelerate the loss of firmness. Taking into account the theory of free radical or telomeric aging, it may be stated that the skin exposed to extreme conditions ages faster. When faced with such a problem, it seems pointless to apply invasive rejuvenating treatments without prior diagnosis or acquiring basic knowledge of care and hygiene of the epidermis. Daily skin care and cosmetics play a key role in the anti-aging process. Hygiene and proper cosmetic habits are essential for health and youthful appearance of the skin. Appropriate regulatory exfoliation, or the so-called turnover of the epidermis, should be used so as not to generate excessive free radical stress. Antioxidants are necessary for this. Modern products based on a liquid crystal base compatible with the liquid crystal structure of epidermal cement together with active emollients allow for restoration of appropriate epidermis parameters. The method of exfoliation with selected ingredients enables long-term skin cleansing by accelerating the turnover of the epidermis without excessive free radical stress, drying and irritation.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontierspartnerships.org/articles/10.3389/abp.2026.15484</guid>
        <link>https://www.frontierspartnerships.org/articles/10.3389/abp.2026.15484</link>
        <title><![CDATA[Genetic architecture of obesity and advances in precision pharmacotherapy: a comprehensive review]]></title>
        <pubdate>2026-04-07T00:00:00Z</pubdate>
        <category>Review</category>
        <author>Floren Kavaja</author><author>Thomas Liehr</author><author>Gazmend Temaj</author>
        <description><![CDATA[Obesity, a global health catastrophe, arises from complex interactions between environmental factors and genetic predispositions. This review summarizes the current state of knowledge on the genetic basis of obesity and contrasts rare monogenic forms caused by mutations in a single gene with common polygenic forms caused by hundreds of genetic variants with small effects. We highlight important genes in neuroendocrine signaling pathways, particularly the leptin-melanocortin system involving MC4R, LEP, and POMC, as well as newly identified loci from genome-wide association studies such as FTO and SEC16B. The interplay between genetic probability and environmental factors underscores the heterogeneity of obesity phenotypes. Recent advances in pharmacotherapy, such as GLP-1 receptor agonists and dual/triple incretin agonists, demonstrate strong efficacy across various genetic backgrounds and underscore the translational relevance of genetic insights. New findings from different groups support the use of polygenic risk scores to identify individuals at risk and suggest prevention strategies. This review discusses the genomic data on clinical practice and emphasizes the possibilities and challenges of precision medicine in obesity treatment. Future research should focus on length of genetic screening and elucidating gene-environment interactions to optimize treatment outcomes.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontierspartnerships.org/articles/10.3389/abp.2026.15939</guid>
        <link>https://www.frontierspartnerships.org/articles/10.3389/abp.2026.15939</link>
        <title><![CDATA[Species barriers in AAV tropism: mechanisms, models, and emerging solutions for clinical translation]]></title>
        <pubdate>2026-03-26T00:00:00Z</pubdate>
        <category>Mini Review</category>
        <author>Xinyuan Xu</author>
        <description><![CDATA[Adeno-associated virus (AAV) vectors have become central to in vivo gene therapy across genetic and acquired diseases. Yet despite extensive preclinical validation, AAV programs often encounter translational gaps when advancing from rodents to non-human primates (NHPs) and ultimately to humans. These species barriers arise from differences in capsid–receptor interactions, intracellular trafficking, immune landscapes, and tissue microanatomy. Even within species, strain-level genetic variation can markedly alter vector performance, exemplified by the LY6A-dependent central nervous system (CNS) tropism of AAV-PhP.B in C57BL/6J mice. Old world monkeys, which are evolutionarily closer to humans than new world species, remain the most widely used models for systemic and CNS delivery, yet discrepancies in seroprevalence, complement activity, endothelial biology, and neuronal susceptibility still limit predictability. Recent advances, including machine learning–guided capsid design, deep mutational scanning, and human-derived organoids and explant models, offer powerful tools to bridge these barriers. This mini-review synthesizes current understanding of AAV species barriers and outlines strategies that enhance the robustness and human relevance of AAV gene therapy development.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontierspartnerships.org/articles/10.3389/abp.2026.16188</guid>
        <link>https://www.frontierspartnerships.org/articles/10.3389/abp.2026.16188</link>
        <title><![CDATA[Association between systemic inflammatory markers and recurrence risk in benign paroxysmal positional vertigo: a retrospective cohort study]]></title>
        <pubdate>2026-03-23T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Bekir Doğan</author><author>Deniz Baklacı</author>
        <description><![CDATA[ObjectiveBenign paroxysmal positional vertigo (BPPV) is a common vestibular disorder that responds well to canalith repositioning maneuvers but is frequently complicated by recurrence. Increasing evidence suggests that systemic inflammation may influence disease course and recurrence risk. This study aimed to investigate the association between routinely available systemic inflammatory markers and BPPV recurrence within 6 months.MethodsIn this single-center retrospective cohort study, 300 adult patients diagnosed with BPPV between January 2020 and December 2024 were included. Neutrophil-to-lymphocyte ratio (NLR), systemic immune-inflammation index (SII), and C-reactive protein (CRP) levels measured at initial presentation were analyzed. Recurrence within 6 months following successful canalith repositioning maneuvers was defined as the primary outcome. Group comparisons were performed using non-parametric tests. Independent predictors of recurrence were identified using multivariable logistic regression analysis, and receiver operating characteristic (ROC) analysis was used to evaluate discriminatory performance.ResultsDuring the six-month follow-up period, recurrence occurred in 78 patients (26.0%). Patients with recurrence had significantly higher NLR and CRP levels compared with those without recurrence (both p < 0.001). SII values were also elevated in the recurrence group, although the difference was less pronounced (p = 0.048). In multivariable analysis adjusted for age, sex, and canal involvement, both NLR (odds ratio [OR]: 1.42; 95% confidence interval [CI]: 1.18–1.71) and CRP (OR: 1.27; 95% CI: 1.10–1.46) emerged as independent predictors of BPPV recurrence. ROC analysis demonstrated moderate discriminatory ability for NLR (AUC = 0.71) and CRP (AUC = 0.69), whereas SII showed limited predictive performance (AUC = 0.62).ConclusionSystemic inflammatory markers, particularly NLR and CRP, are independently associated with recurrence risk in BPPV. Given their low cost and widespread availability, these markers may provide complementary information for early risk stratification. Further prospective and multicenter studies are required to clarify causal mechanisms and confirm clinical applicability.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontierspartnerships.org/articles/10.3389/abp.2026.16199</guid>
        <link>https://www.frontierspartnerships.org/articles/10.3389/abp.2026.16199</link>
        <title><![CDATA[Differential in vitro and in vivo responses of Akkermansia muciniphila to Odontosoria biflora (Kaulf.) C.Chr. [Lindsaeaceae] hexane extract in diet- and alloxan-induced BALB/c mice]]></title>
        <pubdate>2026-02-24T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Marvie Hamel Darbandi</author><author>Leslie Michelle M. Dalmacio</author>
        <description><![CDATA[Akkermansia muciniphila is a mucin-degrading gut bacterium linked to metabolic health, yet culture-based growth stimulation may not translate to sustained enrichment in vivo. Odontosoria biflora (“tubho”) is traditionally consumed in the Philippines as a herbal tea, but its extract-level activity toward A. muciniphila remains poorly characterized. O. biflora was sequentially extracted (hexane, ethyl acetate, methanol, aqueous) and screened for growth-supporting activity toward A. muciniphila in modified BHI under anaerobic conditions. The most active fraction (hexane; OBE HEX) was evaluated for acute oral tolerability in BALB/c mice according to OECD Tesy No. 423 (up to 2000 mg/kg) and subsequently assessed in a high-fat/high-sugar diet plus alloxan-induced diabetic model. Fecal A. muciniphila-specific qPCR signal was monitored at weeks 0, 1, 2, and 4 using a modified 2−ΔΔCt approach with external ATCC genomic DNA as a reference. OBE-HEX produced the strongest in vitro growth-supporting effect (56.43% at 250 mg/L, p < 0.05; 85.62% at 500 mg/L, p < 0.001) and showed no observable toxicity in vivo. In contrast, in vivo analysis revealed only transient changes in fecal A. muciniphila detection following OBE-HEX administration, whereas sustained elevation was observed only in metformin-treated mice. Untargeted UPLC-ESI-QTOF-MS analysis of OBE-HEX yielded putative identification of 2-O-rhamnosylvitexin and 7-methoxy-9,10-dihydrophenanthrene-2,5-diol. Overall, these findings demonstrate that while O. biflora hexane extract exhibits direct growth-supporting activity toward A. muciniphila in vitro and is orally tolerable, such effects do not translate into sustained in vivo enrichment under diabetic conditions, underscoring the limitations of extrapolating culture-based microbiota screening results to host-associated systems.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontierspartnerships.org/articles/10.3389/abp.2026.15861</guid>
        <link>https://www.frontierspartnerships.org/articles/10.3389/abp.2026.15861</link>
        <title><![CDATA[Structural potential of the 5′ noncoding regions of the mRNAs encoding p53 isoforms]]></title>
        <pubdate>2026-02-19T00:00:00Z</pubdate>
        <category>Mini Review</category>
        <author>Mariola Dutkiewicz</author><author>Paulina Zydowicz-Machtel</author>
        <description><![CDATA[Famous for its nickname “guardian of the genome,” the p53 protein acts, among other things, as a transcription factor in the form of a tetramer, which may consist of different types of p53 isoforms. They differ in length and content of specific domains that are responsible for their functions. The way this factor acts, sometimes opposite to what we would expect from the main protein isoform, depends on which isoforms form the tetramer. There are over a dozen isoforms of the human p53 protein encoded by a single gene, thanks to the use of different transcriptional promoters (DNA level), alternative splicing (pre-mRNA level), and different translation initiation sites (mRNA level). In vitro studies have demonstrated that the use of different translation initiation sites on full-length p53FL mRNA is possible due to specific RNA structures, and that these structures are also responsible for the rate and efficiency of target protein isoform formation. This affects the proportions between the different p53 isoforms present in the cell at a given moment and, consequently, the further fate of the cell. This paper summarizes the knowledge about the importance of the RNA structure (I-III order) of individual p53 transcripts for the fate of the cell and the organism.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontierspartnerships.org/articles/10.3389/abp.2026.15244</guid>
        <link>https://www.frontierspartnerships.org/articles/10.3389/abp.2026.15244</link>
        <title><![CDATA[Bibliometric analysis of research trends on the epiretinal membrane since 2000]]></title>
        <pubdate>2026-02-12T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>ShuHan Zeng</author><author>Rui Wang</author><author>Jie Chen</author><author>Yue He</author>
        <description><![CDATA[Epiretinal membrane (ERM) is an important retinal disorder, yet its global research landscape has not been systematically evaluated. This study conducted a comprehensive bibliometric analysis of ERM-related publications indexed in the Web of Science Core Collection. Data were analyzed using CiteSpace, bibliometrix, VOSviewer, and the bibliometrix R package to assess publication trends, international collaboration, influential journals, and evolving research topics. A total of 1,059 articles published between 2000 and 2024 were included. The annual number of publications showed a steady increase, with the United States and other developed countries contributing the majority of research output. Journal analysis identified Retina-The Journal of Retinal and Vitreous Diseases as the leading outlet in terms of productivity. Keyword co-occurrence and co-citation analyses revealed that current research hotspots involve epidemiology, molecular mechanisms, imaging biomarkers associated with surgical outcomes, and the emerging use of artificial intelligence in diagnosis and prognosis prediction. These findings provide an integrated overview of ERM research development, highlight major thematic shifts, and offer valuable guidance for future investigations in this field.]]></description>
      </item>
      </channel>
    </rss>