TY - JOUR AU - Mawad, Habib AU - Pinard, Louis AU - Medani, Samar AU - Chagnon, Miguel AU - Boucquemont, Julie AU - Turgeon, Julie AU - Dieudé, Mélanie AU - Hamelin, Katia AU - Rimbaud, Annie Karakeussian AU - Belayachi, Ali AU - Yang, Bing AU - Collette, Suzon AU - Sénécal, Lynne AU - Foster, Bethany J. AU - Hébert, Marie-Josée AU - Cardinal, Héloïse PY - 2023 M3 - Original Research TI - Hypothermic Perfusion Modifies the Association Between Anti-LG3 Antibodies and Delayed Graft Function in Kidney Recipients JO - Transplant International UR - https://www.frontierspartnerships.org/articles/10.3389/ti.2023.10749 VL - 36 SN - 1432-2277 N2 - We previously reported associations between autoantibodies to the LG3 fragment of perlecan, anti-LG3, and a higher risk of delayed graft function (DGF) in kidney transplant recipients. Here, we aimed to determine whether some factors that modulate ischemia-reperfusion injury (IRI) can modify this association. We performed a retrospective cohort study in kidney transplant recipients in 2 university-affiliated centers. In 687 patients, we show that high pre-transplant anti-LG3 are associated with DGF when the kidney is transported on ice (odds ratio (OR): 1.75, 95% confidence interval 1.02–3.00), but not when placed on hypothermic perfusion pump (OR: 0.78, 95% CI 0.43–1.37). In patients with DGF, high pre-transplant anti-LG3 are associated with a higher risk of graft failure (subdistribution hazard ratio (SHR): 4.07, 95% CI: 1.80, 9.22), while this was not the case in patients with immediate graft function (SHR: 0.50, 95% CI 0.19, 1.29). High anti-LG3 levels are associated with a higher risk of DGF in kidneys exposed to cold storage, but not when hypothermic pump perfusion is used. High anti-LG3 are also associated with a higher risk of graft failure in patients who experience DGF, a clinical manifestation of severe IRI. ER -